Isolation and Long-term Cultivation of Mouse Alveolar Macrophages
نویسندگان
چکیده
منابع مشابه
the effects of keyword and context methods on pronunciation and receptive/ productive vocabulary of low-intermediate iranian efl learners: short-term and long-term memory in focus
از گذشته تا کنون، تحقیقات بسیاری صورت گرفته است که همگی به گونه ای بر مثمر ثمر بودن استفاده از استراتژی های یادگیری لغت در یک زبان بیگانه اذعان داشته اند. این تحقیق به بررسی تاثیر دو روش مختلف آموزش واژگان انگلیسی (کلیدی و بافتی) بر تلفظ و دانش لغوی فراگیران ایرانی زیر متوسط زبان انگلیسی و بر ماندگاری آن در حافظه می پردازد. به این منظور، تعداد شصت نفر از زبان آموزان ایرانی هشت تا چهارده ساله با...
15 صفحه اولIsolation and long-term culture of neural stem cells from chondrostei fish Acipenser persicus
In the present study, an in vitro brain cell culture was developed from Persian sturgeon (Acipenser persicus). The tissues from anterior, middle and posterior regions of the brain were dissected, dispersed and cultivated separately in DMEM/F12 medium supplemented with 15% fetal bovine serum, antibiotic and antimycotic. The medium was changed every 3 days. The cells became confluent after about ...
متن کاملMouse leukemia virus: "spontaneous" release by mouse embryo cells after long-term in vitro cultivation.
BALB/c mouse embryo cells maintained in tissue culture on a schedule of rapid transfer at high cell density develop into tumorigenic lines that have lost contact inhibition of cell division. After several months in culture, certain of these lines begin to release mouse leukemia virus. This virus has properties indistinguishable from those of virus found in adult BALB/c mice. The evidence presen...
متن کاملIsolation and long-term culture of neural stem cells from Acipenser persicus (Borodin, 1897)
In the present study, an in vitro brain cell culture was developed from neural cells of Persian sturgeon (Acipenser persicus). The tissue samples collected from the anterior, middle and posterior regions of the brain were cultivated separately in DMEM/F12 medium supplemented with 15% fetal bovine serum, antibiotic and antimycotic. The medium was refreshed every 3 days. The cells became confluen...
متن کاملCHROMOSOMAL PROTEINS IN PULMONARY ALVEOLAR MACROPHAGES
In this study the nature of chromosomal proteins, his tones and nonhistone in resident alveolar macrophages was investigated in comparison to peritoneal neutrophils and calf thymus proteins. Cells were obtained by lavaging the lung and after purity determination they were subjected to fractional extraction procedures. Proteins were then analysed on SDS polyacrylamide gels and densitometric...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2019
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3302